CEPHALINS, CHOLINE-CONTAINING PHOSPHOLIPIDS, AND TOTAL PHOSPHOLIPIDS IN NORMAL HUMAN PLASMA
نویسندگان
چکیده
منابع مشابه
Semiautomated spectrophotometry of total phospholipids in plasma.
A one-stage semiautomated method for estimating total phospholipids in plasma is reported. The phospholipids are extracted into aqueous ethanol, combined with a Lewis acid, and the change in pH is monitored colorimetrically. The method, compared with the acid digestion method of Gomori [J. Lab. Clin. Med. 27, 955 (1942)], gives results that are about 0.25 mmol/liter lower, but seems to be a sui...
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An enzymatic method, previously used to determine choline-containing phospholipids in serum, was applied to the estimation of phospholipid concentration in bile. The method combines three enzymatic reactions: a) release of choline by phospholipase D; b) choline oxidation by choline oxidase, a reaction which generates hydrogen peroxide; and c) formation of a red quinone dye by peroxidase. The me...
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The use of reineckate as a precipitating agent in the determination of choline was introduced by Kapfhammer and Bischoff (1). This precipitation was later adapted by Beattie to the calorimetric determination of micro quantities of choline (2). Beattie used a saturated solution of ammonium reineckate in water for the precipitation of choline and stated that the precipitation of choline at temper...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1941
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(19)51363-1